※ Some information (<USERNAME>
, <PASSWORD>
, <SSLURL>
) is hidden due to "security issues".
from gummy import TranslationGummy
from gummy.utils import get_driver
[success] local driver can be built. [failure] remote driver can't be built. DRIVER_TYPE: local
with get_driver() as driver:
model = TranslationGummy(driver=driver, gateway="utokyo", translator="deepl")
pdf_path = model.toPDF(url="https://academic.oup.com/brain/article/142/7/1905/5506062")
print(pdf_path)
DRIVER_TYPE: local Estimated Journal Type : OxfordAcademic Crawling Type: soup Use UTokyoGateWay._pass2oxfordacademic method. Fill <USERNAME> in element with id=username Fill <PASSWORD> in element with id=password Click the element with id=btnSubmit_6 Click the element with id=btnContinue Get <SSLURL>/brain/article/142/7/1905/5506062 (wait 0[s] for loading..) Decompose unnecessary tags to make it easy to parse. ============================== Decomposed <i> tag (46) Decomposed <link> tag (21) Decomposed <meta> tag (160) Decomposed <noscript> tag (3) Decomposed <script> tag (43) Decomposed <style> tag (12) Decomposed <sup> tag (12) Decomposed <None> tag (0) Show contents of the paper ============================== [001/124] Abstract [002/124] Allele-specific silencing by... [003/124] Introduction [004/124] Autosomal dominant adult-onset... [005/124] At the cellular... [006/124] In recent years,... [007/124] However, a major... [008/124] Other than a... [009/124] In this study,... [010/124] Compared to other... [011/124] Since ADLD patients... [012/124] Figure 1 [013/124] Overview of the... [014/124] Overview of the... [015/124] To the best... [016/124] Materials and methods [017/124] Plasmid vectors are... [018/124] Cell lines and cell culture [019/124] Fibroblast cell lines... [020/124] The HEK293T cells... [021/124] Primary oligodendrocyte precursor... [022/124] Design of siRNAs and generation of shRNA recombinant lentivirus particles [023/124] We designed an... [024/124] The most efficient... [025/124] Dual luciferase reporter assay [026/124] HEK293T cells were... [027/124] Validation of allele-specific siRNAs in ADLD fibroblasts [028/124] ADLD and control... [029/124] To evaluate the... [030/124] Direct reprogramming of ADLD and control human fibroblasts in neurons [031/124] Viral production and titration [032/124] HEK293NT cells were... [033/124] The virus was... [034/124] Direct neuronal reprogramming [035/124] Low-passage primary human... [036/124] Validation of allele-specific shRNAs in ADLD neurons [037/124] Cells were transduced... [038/124] Validation of allele-specific shRNAs in rat oligodendrocytes cultures overexpressing human LMNB1 coding sequence [039/124] After the shaking... [040/124] RNA isolation and quantitative real time PCR [041/124] Total RNA was... [042/124] Reactions were carried... [043/124] Allelic discrimination by primer extension assay [044/124] To evaluate the... [045/124] Western blot analysis [046/124] Total protein was... [047/124] Immunofluorescence [048/124] Fibroblasts [049/124] Cells were plated... [050/124] Reprogrammed neurons and primary oligodendrocyte precursors cell cultures [051/124] Reprogrammed neurons and... [052/124] In a set-up... [053/124] Note that because... [054/124] Upon transfection with... [055/124] Statistical analysis [056/124] Graphics and statistical... [057/124] Data availability [058/124] The authors confirm... [059/124] Results [060/124] Identification of allele-specific siRNAs [061/124] To exploit the... [062/124] Bioinformatics tools that... [063/124] Figure 2 [064/124] ASP-siRNA screening. (A)... [065/124] ASP-siRNA screening. (A)... [066/124] The results are... [067/124] ASP-siRNAs restored physiological LMNB1 levels and ameliorated ADLD cellular phenotypes in patient fibroblasts [068/124] Human ADLD fibroblasts... [069/124] We evaluated the... [070/124] Figure 3 [071/124] Primary readouts of... [072/124] Primary readouts of... [073/124] We accurately evaluated... [074/124] Next, we verified... [075/124] Furthermore, to evaluate... [076/124] By fluorescent microscopy,... [077/124] Figure 4 [078/124] Secondary readouts of... [079/124] Secondary readouts of... [080/124] To evaluate transcriptome... [081/124] ASP-siRNA treatment ameliorated ADLD-specific cellular phenotypes in disease-relevant cellular models [082/124] Disease-relevant cellular and... [083/124] Validation of the therapeutic potential of ASP-siRNA in reprogrammed ADLD neurons [084/124] Fibroblasts from three... [085/124] To validate our... [086/124] Figure 5 [087/124] Validation of ASP-RNAi... [088/124] Validation of ASP-RNAi... [089/124] Validation of the therapeutic potential of ASP-siRNA in rat oligodendrocyte cultures overexpressing hLMNB1 [090/124] Primary OPCs were... [091/124] OPCs overexpressing the... [092/124] Figure 6 [093/124] Validation of ASP-RNAi... [094/124] Validation of ASP-RNAi... [095/124] Discussion [096/124] ADLD is a... [097/124] As ADLD is... [098/124] We reasoned that... [099/124] ADLD patients have... [100/124] Identification of efficient... [101/124] Thus, we planned... [102/124] Beyond their structural... [103/124] Overall, our results... [104/124] To demonstrate ASP-silencing... [105/124] Both cellular models... [106/124] The treatment reduced... [107/124] ADLD is a... [108/124] Following these results,... [109/124] To overcome this... [110/124] Delivery of ASP-siRNAs... [111/124] Our work represents... [112/124] Genetic diseases associated... [113/124] Question marks indicate... [114/124] Genetic diseases associated... [115/124] Question marks indicate... [116/124] Overall, our work... [117/124] autosomal dominant adult-onset... [118/124] allele-specific silencing by... [119/124] oligodendrocyte precursor cell... [120/124] single nucleotide polymorphism... [121/124] We thank Dr... [122/124] The work was... [123/124] A.Br. and E.G.... 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1.083[s] [131/136] (query1) 02/20 [##------------------] 10.00% - 2.189[s] [132/136] (query1) 01/20 [#-------------------] 5.00% - 1.087[s] [133/136] (query1) 02/20 [##------------------] 10.00% - 2.253[s] [134/136] (query1) 04/20 [####----------------] 20.00% - 4.330[s] [135/136] (query1) 02/20 [##------------------] 10.00% - 2.173[s] Save HTML file at /Users/iwasakishuto/.gummy/Allele-specific silencing as treatment for gene duplication disorders: proof-of-principle in autosomal dominant leukodystrophy.html Convert from HTML to PDF ============================== Loading pages (1/6) Counting pages (2/6) Resolving links (4/6) Loading headers and footers (5/6) Printing pages (6/6) Done Save PDF file at /Users/iwasakishuto/.gummy/Allele-specific silencing as treatment for gene duplication disorders: proof-of-principle in autosomal dominant leukodystrophy.pdf Delete original HTML file at /Users/iwasakishuto/.gummy/Allele-specific silencing as treatment for gene duplication disorders: proof-of-principle in autosomal dominant leukodystrophy.html /Users/iwasakishuto/.gummy/Allele-specific silencing as treatment for gene duplication disorders: proof-of-principle in autosomal dominant leukodystrophy.pdf